Trapping of Hepatitis B Virus Capsid Assembly Intermediates by Phenylpropenamide Assembly Accelerators
نویسندگان
چکیده
منابع مشابه
Hepatitis B virus capsid assembly Assembly pathway of hepatitis B core virus-like particles from genetically fused dimers
Background: We studied assembly of HBV capsids using a genetically-fused dimer. Results: Assembly intermediates composed of two, three and five copies of a fused dimer have been isolated. Conclusion: Capsid assembly could progress using dimeric, trimeric and pentameric intermediates. Significance: The low abundance and transient nature of assembly intermediates make analysis challenging, howeve...
متن کاملRunning title: Targeting virus assembly Small-Molecule Effectors of Hepatitis B Virus Capsid Assembly Give Insight Into Virus Lifecycle
word count: 203 d – current address 250 McElroy Hall Department of Veterinary Pathobiology Oklahoma State University Stillwater, Oklahoma 74078-2007 e* current address, corresponding author Adam Zlotnick Department of Biology Indiana University Simon Hall MSB, Room 220D 212 S. Hawthorne Drive Bloomington IN 47405-7003 [email protected] fax 812-856-5710 AC CE PT ED Copyright © 2008, American ...
متن کاملA Kinase Chaperones Hepatitis B Virus Capsid Assembly and Captures Capsid Dynamics in vitro
The C-terminal domain (CTD) of Hepatitis B virus (HBV) core protein is involved in regulating multiple stages of the HBV lifecycle. CTD phosphorylation correlates with pregenomic-RNA encapsidation during capsid assembly, reverse transcription, and viral transport, although the mechanisms remain unknown. In vitro, purified HBV core protein (Cp183) binds any RNA and assembles aggressively, indepe...
متن کاملHepatitis B virus capsid assembly is enhanced by naturally occurring mutation F97L.
In chronic hepatitis B virus (HBV) infections, one of the most common mutations to the virus occurs at amino acid 97 of the core protein, where leucine replaces either phenylalanine or isoleucine, depending on strain. This mutation correlates with changes in viral nucleic acid metabolism and/or secretion. We hypothesize that this phenotype is due in part to altered core assembly, a process requ...
متن کاملSmall-molecule effectors of hepatitis B virus capsid assembly give insight into virus life cycle.
The relationship between the physical chemistry and biology of self-assembly is poorly understood, but it will be critical to quantitatively understand infection and for the design of antivirals that target virus genesis. Here we take advantage of heteroaryldihydropyrimidines (HAPs), which affect hepatitis B virus (HBV) assembly, to gain insight and correlate in vitro assembly with HBV replicat...
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ژورنال
عنوان ژورنال: ACS Chemical Biology
سال: 2010
ISSN: 1554-8929,1554-8937
DOI: 10.1021/cb100275b